Sclerotinia Initiative Activity Report – August 2005 – 2006
a) Title of the agreement:
Mapping and Transfer of Sclerotinia Resistance from Scarlet Runner to Common Bean
James R. Myers
Department of Horticulture, ALS 4017
Oregon State University
Corvallis, OR 97331
b) Significant findings made during the past year:
- We participated in greenhouse straw test and field trial of the National White Mold Trial. Straw test results were reported in the 2004-2005 activity report. Field scores were reported to University of Nebraska and have been compiled with their report.
- 91G x PI255956 BC2F4 population (a cross between a white mold susceptible P. vulgaris cultivar and a resistant P. coccineus accession) was sampled for genotyping, evaluated for field resistance by families and advanced to BC2F5 at the OSU vegetable farm. 115 BC2F5 lines were then subjected to three straw tests and an oxalate test in the greenhouse and advanced to BC2F6. The straw tests revealed significant differences between lines with many lines less susceptible than the recurrent parent, a few lines transgressively more susceptible than the recurrent parent, and some lines no different in response from the resistant check G122. While the three straw tests are well correlated (p<0.005), they show no significant correlation with oxalate scores (p=0.83, 0.91, 0.22). This may indicate that additional factors in addition to oxalate sensitivity may condition physiological resistance. Currently, these lines are being genotyped. We have identified 45 SSR loci polymorphic in the population and have scored 12 so far.
- 91G x PI433251B, MO162 x PI433251B, and G122 x PI433251B were advanced in the field to form BC2F2 segregating populations. 91G x PI433251B is intended to integrate a second source of resistance from P. coccineus into susceptible common beans. It may provide additional validation for identified QTL for resistance found in the 91G x PI255956 population. MO162 x PI433251B and G122 x PI433251B pyramid putatively different sources of resistance from P. vulgaris and P. coccineus into a single package. These populations will help us answer the question of whether resistances in MO162 and G122 are allelic to that found in P. coccineus, and whether pyramiding produces higher levels of resistance than the original sources.
c) List of any publications/patents related to the agreement:
New publications:
Haggard, J. E. and J. R. Myers. 2006. Characterization of Physiological Resistance to White Mold and Search for Molecular Markers Linked to Resistance via Advanced Backcross QTL Analysis in an Interspecific Cross between Phaseolus coccineus and P. vulgaris. HortScience 41:(Abstract, In Press).
Haggard, J. E. and J. R. Myers. 2006. Further progress in transferring Sclerotinia resistance from runner bean to common bean. (Poster Sclerotinia Initiative meeting, Minneapolis, MN, January, 2006.)
Publications reported in previous annual reports:
Myers, J. R. and J. E. Haggard. 2005. Progress in transferring Sclerotinia resistance from runner bean to common bean. (Poster Sclerotinia Initiative meeting, Minneapolis, MN, January, 2005.)
Chipps, T.J., B. Gilmore, J. Myers, H.U. Stotz. 2005. Evidence for oxalate insensitivity and oxalate oxidase in determining partial resistance of Phaseolus coccineus to Sclerotinia sclerotiorum. Phytopathology 95: 292-299.
Gilmore, B., and J.R. Myers. 2004. A Preliminary Molecular Marker Map for Phaseolus coccineus. Annu. Rept. Bean Improv. Coop. 47:87-88.
Steadman, J. R., L. K. Otto-Hanson, K. Powers, C. Kurowski, R. Mainz, J. Kelly, P. Griffiths, K. Grafton, J. Myers, P. Miklas and H. Schwartz. 2004. Identification of partial resistance to Sclerotinia sclerotiorum in common bean at multiple locations. Ann. Rep. Bean Improv. Coop. 47:281-282.
Chipps, T.J., B. Gilmore, J.R. Myers, and H.U. Stotz. 2004. Genetic and physiological basis of partial resistance to Sclerotinia sclerotiorum in Phaseolus coccineus. Phytopathology 94:S19. (Abstract).
Stotz, H.U., T.J. Chipps, B. Gilmore, and J.R. Myers. 2004. Genetic and Physiological Basis of Partial Resistance to Sclerotinia sclerotiorum in Phaseolus coccineus. American Society of Plant Biologists Annual Meeting. Abstract No. 532.
