Annual Report: 2005

Development and Use of DNA Markers for the Control of Sclerotinia in Sunflower

Principal Investigator:
Dr. Jinguo Hu
USDA-ARS-RRVARC
Fargo, ND

2006 Annual Report:
This report serves to document research conducted under a specific cooperative agreement between ARS and North Dakota State University, Department of Plant Sciences.  Additional details of research can be found in the report for the parent project 5442-22000-027-00D “Enhancement of Sunflower Germplasm Diversity through the Use of Wild Species and Genome Characterization."

One hundred and twenty-three F2:3 lines derived from the cross of HA 441 x RHA 439 were grown in two locations for mapping quantitative trait loci (QTLs) associated with head rot resistance in this summer.  We have extracted the DNA and genotyped each line using both TRAP and SSR markers.  Ninety-three lines of a RIL population derived from the cross of RHA280 x RHA 801 were used for mapping QTL for stalk rot resistance in this summer.  We have finished one round of stalk rot resistance test in greenhouse by artificial inoculation, and these lines were also grown in two locations in this summer for stalk rot resistance investigation.

Contact Information:
Dr. Jinguo Hu, Research Molecular Geneticist
USDA-ARS-RRVARC
Northern Crop Science Laboratory
Sunflower Research Unit
1307 18th Street North
Fargo, ND  58105-5677
701-239-1351